Journal: Stem Cell Research & Therapy

Article Title: Mesenchymal stem/stromal cells overexpressing leukemia inhibitory factor (LIF) promote arteriogenesis and functional recovery in a mouse model of critical hindlimb ischemia

doi: 10.1186/s13287-025-04762-z

Figure Lengend Snippet: Pro-angiogenic potential of MSC_LIF in vivo. A Matrigel plugs containing MSC or MSC_LIF conditioned medium or 0.9% saline solution (- CTL) after excision of the mouse ventral area ( n = 4 animals per group). B Quantification of hemoglobin (Hb) content inside the plugs using Drabkin’s reagent. C Indirect immunofluorescence of sections of paraffin-embedded Matrigel plugs labeled with antibodies against the endothelial marker CD31 (red) and the smooth muscle cell marker αSMA (green). Cell nuclei stained with DAPI (blue). Images obtained by laser confocal microscopy. Scale bars = 100 μm. D Quantification of the mean diameter of capillaries (CD31 + vessels) by area. E Quantification of the mean diameter of arterioles (vessels simultaneously CD31/ αSMA + ) by area. The one-way ANOVA test and the Bonferroni post-test were used to analyze statistical differences. Values expressed as mean ± SEM of two independent experiments. *** p < 0.001; ** p < 0.01

Article Snippet: Tissues were stained with goat anti-mouse CD31 antibody (R&D Systems, diluted 1:100 in 1% BSA Triton-X 0.3% solution) overnight at 4 °C, followed by incubation with Alexa FluorTM 568-conjugated chicken anti-goat antibody (Invitrogen - Thermo Fisher Scientific, diluted 1:1000 in 1x PBS), and Alexa FluorTM 488-conjugated rat anti-mouse αSMA antibody (eBioscience - Thermo Fisher Scientific, diluted 1:400 in 1x PBS) for 1 h at room temperature.

Techniques: In Vivo, Saline, Immunofluorescence, Labeling, Marker, Staining, Confocal Microscopy

Journal: Stem Cell Research & Therapy

Article Title: Mesenchymal stem/stromal cells overexpressing leukemia inhibitory factor (LIF) promote arteriogenesis and functional recovery in a mouse model of critical hindlimb ischemia

doi: 10.1186/s13287-025-04762-z

Figure Lengend Snippet: Assessment of blood vessel distribution in the GST muscle. A Indirect immunofluorescence of cryopreserved sections of GST muscle stained with antibodies against the endothelial marker CD31 (yellow) and the smooth muscle cell marker SMA (green). Cell nuclei were stained with DAPI (blue), and muscle cells were stained with phalloidin (red) ( n = 5 animals per group). Images obtained by laser confocal microscopy. Scale bars = 100 μm. B Quantification of mean capillary length/diameter (CD31 + vessels) per area. C Quantification of mean arteriole length/diameter (vessels simultaneously CD31/αSMA+). The Kruskal-Wallis test and the Dunn’s Multiple Comparison post-test were used to analyze statistical differences. Values expressed as mean ± SEM of three independent experiments. *** p < 0.001; ** p < 0.01; * p < 0.05. The NAIVE group corresponds to the non-induced paw

Article Snippet: Tissues were stained with goat anti-mouse CD31 antibody (R&D Systems, diluted 1:100 in 1% BSA Triton-X 0.3% solution) overnight at 4 °C, followed by incubation with Alexa FluorTM 568-conjugated chicken anti-goat antibody (Invitrogen - Thermo Fisher Scientific, diluted 1:1000 in 1x PBS), and Alexa FluorTM 488-conjugated rat anti-mouse αSMA antibody (eBioscience - Thermo Fisher Scientific, diluted 1:400 in 1x PBS) for 1 h at room temperature.

Techniques: Immunofluorescence, Staining, Marker, Confocal Microscopy, Comparison